Products
FOXA2 antibody
- Product Name
- FOXA2 antibody
- Catalogue No.
- FNab03188
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- forkhead box A2
- Alternative Names
- forkhead box A2 antibody, Forkhead box protein A2 antibody, FOXA2 antibody, HNF 3 beta antibody, HNF 3B antibody, HNF3B antibody, TCF 3B antibody, TCF3B antibody, Transcription factor 3B antibody
- UniProt ID
- Q9Y261
- Observed MW
- 60 kDa
Application
- Tested Applications
- ELISA, IP, WB, IHC
- Recommended dilution
- WB: 1:500-1:5000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:50-1:500
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab03188(FOXA2 Antibody) at dilution of 1:1000
IP Result of anti-FOXA2 (IP:FNab03188, 3ug; Detection:FNab03188 1:300) with HepG2 cells lysate 1920ug.
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using FNab03188(FOXA2 Antibody) at dilution of 1:50
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using FNab03188 ( FOXA2 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
- Background
- Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'(By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
